国产亚洲精品福利视频在线观看|无遮挡边摸边吃奶边做视频免费|在办公室拨开老师内裤进入|国产日韩欧美一区二区东京热

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人正常細(xì)胞>CCL-171MRC-5 人胚肺細(xì)胞

MRC-5 人胚肺細(xì)胞

簡(jiǎn)要描述:CCL-171 MRC-5 人胚肺細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CCL-171
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-16
  • 訪  問(wèn)  量:2059

產(chǎn)品分類

Product Category

詳細(xì)介紹

CCL-171 MRC-5 人胚肺細(xì)胞

ATCC® Number:  CCL-171™

Designations:  MRC-5

Depositors:   JP Jacobs

Biosafety Level: 1

Shipped:  frozen

Medium & Serum:  See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: fibroblast

CCL-171 MRC-5 人胚肺細(xì)胞

Source: Organ: lung

Disease: normal

Cell Type: fibroblast

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Isolation:  Isolation date: September, 1966

Applications: efficacy testing [92587]

testing [92346] [92389]

transfection host (Roche FuGENE® Transfection Reagents)

viruscide testing [92579] [92582]

Virus Susceptibility: poliovirus 1; herpes simplex; vesicular stomatitis (Indiana)

Reverse Transcript: negative

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 11,12

D13S317: 11,14

D16S539: 9,11

D5S818: 11,12

D7S820: 10,11

THO1: 8

TPOX: 8

vWA: 15

Cytogenetic Analysis: Chromosome Frequency Distribution 50 Cells: 2n = 46. This is a normal diploid human cell line with 46,XY karyotype. The modal chromosome number was 46, occurring in 70% of cells. The rate of polyploidy was 3.6%. Both X and Y chromosomes were normal.

Isoenzymes:  G6PD, B

Age:  14 weeks gestation

Gender:  male

Ethnicity:  Caucasian


Temperature: 37.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing:  Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37°C.

CCL-171



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
中文在线√天堂| 国产av无码一区二区二三区j| 色婷婷综合久色aⅴ五区最新| 国产人妻aⅴ色偷| 婷婷五月婷婷五月| 亚洲av无码精品色午夜| 欧美乱强伦xxxxx高潮| 一本色道久久88—综合亚洲精品| 国产女人高潮视频在线观看| 日韩乱码人妻无码中文字幕| 麻豆精品国产精华精华液好用吗| 欧美另类videosbestsex日本| 非洲黑人性xxxx精品| 人妻 日韩精品 中文字幕| 性一交一乱一伦一色一情| 亚洲国产精品无码专区影院 | 精品国产乱码久久久久久浪潮| 亚欧美日韩香蕉在线播放视频| 亚洲av成人精品综合| 人妻免费久久久久久久了| 草草网站影院白丝内射| 爱情岛论坛首页永久入口| 一边捏奶头一边高潮视频| 亚洲av乱码乱码精品| 真人无码作爱免费视频| 中文无码热在线视频| 国产喷水福利在线视频| 欧美人与性动交g欧美精器| 日产精品99久久久久久| 欧美性受xxxx黑人xyx性爽| 亚洲一区二区三区 码| 无码人妻丰满熟妇区bbbbxxxx| 爆乳熟妇一区二区三区| 中文字幕无码精品亚洲35| 欧美日韩不卡合集视频| 九九热视频在线精品18| 白又丰满大屁股bbbbb| 欧美孕妇变态孕交粗暴| 日本55丰满熟妇厨房伦| 欧美老妇交乱视频在线观看| 国产一区二区亚洲av|