国产亚洲精品福利视频在线观看|无遮挡边摸边吃奶边做视频免费|在办公室拨开老师内裤进入|国产日韩欧美一区二区东京热

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人正常細(xì)胞>IMR-90人胚肺成纖維細(xì)胞

人胚肺成纖維細(xì)胞

簡要描述:CCL-186 IMR-90 人胚肺成纖維細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號:IMR-90
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-12
  • 訪  問  量:2763

產(chǎn)品分類

Product Category

詳細(xì)介紹

CCL-186 IMR-90人胚肺成纖維細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

CCL-186 IMR-90人胚肺成纖維細(xì)胞.

ATCC® Number: CCL-186™

Designations: IMR-90

Depositors: WW Nichols

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: fibroblast

CCL-186 IMR-90人胚肺成纖維細(xì)胞.

Source: Organ: lung

Disease: normal

Cell Type: fibroblast

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Applications: transfection host (Nucleofection technology from Lonza)

Virus Susceptibility: Human poliovirus 1

Human poliovirus 2

Varicella-Zoster

Herpes simplex virus 1

Herpes simplex virus 2

Human poliovirus 3

Vaccinia virus

Human herpesvirus 5

Vesicular stomatitis virus

Reverse Transcript: negative

DNA Profile (STR): Amelogenin: X

CSF1PO: 11,13

D13S317: 11,13

D16S539: 10,13

D5S818: 12,13

D7S820: 9,12

THO1: 8,9.3

TPOX: 8,9

vWA: 16,19

Cytogenetic Analysis: normal human female; diploid; stable

Isoenzymes: G6PD, B

Age: 16 weeks gestation

Gender: female

Ethnicity: Caucasian

Comments: The human diploid fibroblast strain IMR-90 was derived by W.W. Nichols and associates from the lungs of a 16-week female fetus. [22381]

The division potential, viral susceptibilities and other properties have been thoroughly studied such that the line may be considered as an alternate for WI-38 and other standard human lung cell strains.

The cells have been reported to be capable of attaining 58 population doublings before the onset of senescence. [22381]

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:8 is recommended

Medium Renewal: Every 3 to 4 days

Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

References: 22381: Nichols WW, et al. Characterization of a new human diploid cell strain, IMR-90. Science 196: 60-63, 1977. PubMed: 841339

32932: Dolganov GM, et al. Human Rad50 is physically associated with human Mre11: identification of a conserved multiprotein complex implicated in recombinational DNA repair. Mol. Cell. Biol. 16: 4832-4841, 1996. PubMed: 8756642

33041: Ostlund RE Jr., et al. A sterospecific myo-inositol/D-chiro-inositol transporter in HepG2 liver cells. J. Biol. Chem. 271: 10073-10078, 1996. PubMed: 8626564

Related Links

NCBI Entrez Search

Make a Deposit

Frequently Asked Questions

Material Transfer Agreement

Technical Support

Related Cell Culture Products


IMR-90人胚肺成纖維細(xì)胞更多信息,請致電咨詢。


















 

產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
av天堂永久资源网| 久久777国产线看观看精品| 西西人体www大胆高清| 少妇人妻无码精品视频APP| 国产剧情av麻豆香蕉精品| 老鲁夜夜老鲁| 国产精品久久久久9999小说| 久久99精品久久只有精品| 国产精品无套内射迪丽热巴| 免费看美女被靠到爽的视频| 国产极品尤物久久精品| 中文字幕精品久久久久人妻红杏1 欧美另类videosbestsex日本 | 亚洲精品无码高潮喷水a片软| 国产亚洲精品久久久久久大师| 国产爆乳无码一区二区麻豆| 四房播播网站| 18禁美女裸体免费网站| 久久精品国产99久久久| 丰满五十六十老熟女hd| 日本高清va在线播放| 免费无码又爽又黄又刺激网站| 人妻av中文字幕久久| 日韩精品无码av成人观看| 久久综合九色欧美综合狠狠| 人妻少妇中文字幕久久| 麻麻张开腿让我爽了一夜| 亚洲免费人成在线视频观看| 丰满少妇熟乱xxxxx视频| 搡女人真爽免费视频大全| 欧洲黑大粗无码免费| 国产成人久久综合第一区| 国产女人乱人伦精品一区二区| 国产精品亚洲日韩欧美色窝窝色欲| 国产又黄又爽又色的免费视频| 成人h视频在线观看| 国产香蕉一区二区三区在线视频| 在线国产69自拍视频| 久久精品女人天堂av免费观看| 99久久国产热无码精品免费| 国产免费av片无码永久免费| 日韩人妻ol丝袜av一二区|