在厨房被c到高潮a毛片奶水_伊人天堂av无码av日韩av_午夜亚洲av永久无码精品_欧美xxxx色视频在线观看_国产韩国精品一区二区三区

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>CRL-1676 WM-266-4 人黑素瘤細(xì)胞

CRL-1676 WM-266-4 人黑素瘤細(xì)胞

簡(jiǎn)要描述:CRL-1676 WM-266-4 人黑素瘤細(xì)胞,
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-12
  • 訪  問  量:1825

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-1676 WM-266-4 人黑素瘤細(xì)胞

Designations: WM-266-4

Depositors: M Herlyn

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: epithelial

Source: Organ: skin

Disease: melanoma

Cellular Products: proteoglycan antigen characteristic of melanoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


CRL-1676 WM-266-4 人黑素瘤細(xì)胞

Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X

CSF1PO: 12

D13S317: 12,13

D16S539: 11,12

D5S818: 13

D7S820: 8

THO1: 7,9

TPOX: 8,11

vWA: 15,17

Cytogenetic Analysis: The cell line shares many properties with the parent tumor line including same karyology. This is a hypertriploid human cell line. The modal chromosome number is 82, occurring at 32%, with polyploidy at 8.6%. (Cells with 80 chromosome counts also occurred at a high frequency.) There were two paired [der(1)t(1;?) (p32;?), and der(11)t(2;11) (q22.1;p13)], and three to four single markers consistently found in each cell. Several other markers were also found in some cells only, including a small metacentric chromosome of unknown origin. Neither DMs nor HSRs were detected. Among the normal chromosomes, N11 had only single copy, and N1, N6, N8, N9, and N16 had two copies each. As expected, other normal chromosomes had three to four copies per cell. Two X chromosomes were generally found in each cell.

Age: 58 years

Gender: female

Comments: The WM-266-4 cell line was derived from a metastatic site of a malignant melanoma.

Another cell line derived from the primary tumor of the same patient also is available (ATCC CRL-1675).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol:

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.Add appropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:6 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

derived from same individual:ATCC CRL-1675



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
美丽的熟妇中文字幕| 一本大道人妻中文字幕| 少妇人妻陈艳和黑人教练| 少妇太爽了在线观看免费视频| 无码超乳爆乳中文字幕久久| 中国偷拍老肥熟露脸视频| 久久久久无码精品国产| 久久精品国产亚洲av无码娇色| 国产精品视频永久免费播放| 特大黑人娇小亚洲女| 把女邻居弄到潮喷的性经历| 全程粗话对白视频videos| 英语老师解开裙子坐我腿中间 | 免费观看潮喷到高潮| 婷婷开心五月综合基地| 精品无码一区二区三区的天堂| 亚洲欧洲精品专线| 无码专区一va亚洲v专区在线| 欧美日韩国产综合草草| 日本一区二区国产在线| 国产freexxxx性播放麻豆| 日韩人妻久久中文字幕| 精品无码av一区二区三区不卡| 亚洲人妖一区二区三区| 中文字幕久热精品视频在线| 亚洲综合色成在线观看| 久久久久亚洲av无码专区导航| av国产剧情一区二区三区| 亚洲日本一区二区一本一道| 丝袜人妖av在线一区二区| 中文精品无码中文字幕无码专区| 少妇极品熟妇人妻| 国产乱人伦精品一区二区| 精品久久久久久成人av| 丰满少妇呻吟高潮经历| 国产精品久久国产三级| 成人精品一区二区三区电影| 精品免费国产一区二区三区四区介绍| 无码里番纯肉h在线网站| 三年片在线观看免费观看大全中国 | 人妻无码一区二区三区TV|