在厨房被c到高潮a毛片奶水_伊人天堂av无码av日韩av_午夜亚洲av永久无码精品_欧美xxxx色视频在线观看_国产韩国精品一区二区三区

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>CRL-1504ZR-75-30 人乳腺癌細(xì)胞

ZR-75-30 人乳腺癌細(xì)胞

簡要描述:CRL-1504 ZR-75-30 人乳腺癌細(xì)胞,
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻和培養(yǎng)條件!

  • 產(chǎn)品型號:CRL-1504
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-12
  • 訪  問  量:1995

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-1504  ZR-75-30 人乳腺癌細(xì)胞

ATCC® Number:CRL-1504™Price:
Designations:ZR-75-30Depositors:LW EngelBiosafety Level:1Shipped:frozenMedium & Serum:See PropagationGrowth Properties:adherentOrganism:Homo sapiens (human)Morphology:epithelial Source:Organ: mammary gland; breastDisease: ductal carcinomaDerived from metastatic site: ascitesCell Type: epithelialPermits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.CRL-1504  ZR-75-30 人乳腺癌細(xì)胞DNA Profile (STR):Amelogenin: XCSF1PO: 10,11D13S317: 10,11D16S539: 9D5S818: 12,13D7S820: 11,12THO1: 8,9TPOX: 10,11vWA: 18,19Cytogenetic Analysis:This is a hypertriploid human cell line. The modal chromosome number was 80, occurring in 40% of cells. The rate of cells with higher ploidies was 1.2%. Sixteen marker chromosomes were found in most cells.Age:47 yearsGender:femaleEthnicity:BlackComments:ZR-75-30 was derived from malignant ascites fluid from a 47-year-old premenopausal Black woman with infiltrating ductal carcinoma. These cells have been characterized to be human, non-HeLa, malignant mammary epithelium in origin.Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Atmosphere: air, 95%; carbon dioxide (CO2), 5%Temperature: 37.0°CSubculturing:Protocol:Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell suspension to new culture vessels.Incubate cultures at 37°C.Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:3 is recommendedMedium Renewal: 2 to 3 times per weekPreservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSOStorage temperature: liquid nitrogen vapor phaseDoubling Time:110 hrsRelated Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001recommended serum:ATCC 30-2020purified DNA:ATCC 45532purified DNA:ATCC 45533purified DNA:ATCC CRL-1504DReferences:1230: Engel LW, Young NA. Human breast carcinoma cells in continuous culture: a review. Cancer Res. 38: 4327-4339, 1978. PubMed: 21219323031: Engel LW, et al. Establishment and characterization of three new continuous cell lines derived from human breast carcinomas. Cancer Res. 38: 3352-3364, 1978. PubMed: 68822532275: Littlewood-Evans AJ, et al. The osteoclast-associated protease cathepsin K is expressed in human breast carcinoma. Cancer Res. 57: 5386-5390, 1997. PubMed: 9393764


CRL-1504  


















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
最新国产99热这里只有精品| 成人日韩熟女高清视频一区 | 亚洲精品无码av中文字幕| 亚洲av永久无码精品网站在线观看 | 国内精品久久久久久久小说| 女女互揉吃奶揉到高潮视频| 国产精品福利自产拍在线观看| 男女啪啪做爰高潮免费网站 | 中文字幕第55页一区| 66亚洲一卡2卡新区成片发布| 国产又滑又嫩又白| 97热久久免费频精品99| 日本丰满熟妇videossex| 97精品久久久久中文字幕| 免费观看全黄做爰大片国产| 999久久久免费精品国产| 亚洲av无码乱码国产一区二区| 免费A级毛片樱桃视频| 全国最大成人网| 精品自拍一区国产精品| 人妻无码一区二区三区| 94人妻少妇偷人精品| 久久精品国产精品亚洲艾草网| 亚洲午夜福利在线看片| 亚洲一区二区三区无码中文字幕 | 婷婷五月婷婷五月| 欧美性猛交xxxx黑人猛交| av一本久道久久波多野结衣| 亚洲日韩精品无码专区| 国产又色又爽又黄又免费| 国产丝袜无码一区二区三区视频 | 久久精品人人做人人爽97| 亚洲av无码专区国产乱码电影| 欧美激欧美啪啪片sm| 一本色综合亚洲精品蜜桃冫| 中文在线а√在线| 岛国av大片在线观看| 精品国产污污免费网站| 菠萝蜜视频在线观看入口| 亚洲日韩成人无码| 人妻妺妺窝人体色www聚色窝 |